1.3.3 LncRNA與miRNA結合預測

長(cháng)鏈非編碼RNA（Long-noncoding RNA，lncRNA）是一類(lèi)轉錄本長(cháng)度大于200bp的非編碼RNA，可作為人類(lèi)基因組中一類(lèi)重要的調控分子通過(guò)多種方式發(fā)揮其生物學(xué)功能． 近年來(lái)的研究表明，lncRNA也可以作為一種競爭性?xún)仍葱訰NA（competing endogenous RNA），與miRNA互作結合，形成ceRNA的競爭機制

以下是我們對數據的整理，通過(guò)數據庫查詢(xún)分類(lèi)、數據庫功能及用途、示例結合分析、數據庫優(yōu)化等這四大項，進(jìn)行闡述和演示數據庫的查詢(xún)和使用，希望對您的實(shí)驗項目有所幫助

數據庫查詢(xún)分類(lèi)

① lncRNABase：http://starbase.sysu.edu.cn/mirLncRNA.php

② starbase: http://starbase.sysu.edu.cn/

③ RegRNA2.0：http://regrna2.mbc.nctu.edu.tw/detection.html

數據庫功能及用途：

1. lncRNABase

數據庫功能：

View the predicted miRNA-lncRNA interactions by scanning lncRNA sequences overlapping with CLIP-Seq peaks for potential microRNA targets (miRanda/mirSVR) and then output the detailed information. Here, we list predicted miRNA-target interactions overlapped with CLIP-Seq data

2. starbase：

數據庫功能：

1. starBase is designed for decoding Pan-Cancer and Interaction Networks of lncRNAs, miRNAs, competing endogenous RNAs(ceRNAs), RNA-binding proteins (RBPs) and mRNAs from large-scale CLIP-Seq (HITS-CLIP, PAR-CLIP, iCLIP, CLASH) data and tumor samples (14 cancer types, >6000 samples).

2. starBase is also developed for deciphering Protein-RNA and miRNA-target interactions, such as protein-lncRNA, protein-sncRNA, protein-mRNA, protein-pseudogene, miRNA-lncRNA, miRNA-mRNA, miRNA-circRNA, miRNA-pseudogene, miRNA-sncRNA interactions and ceRNA networks from 108 CLIP-Seq (HITS-CLIP, PAR-CLIP, iCLIP, CLASH) datasets.

3. starBase provides miRFunction and ceRNAFunction web tools to predict the function of ncRNAs (miRNAs, lncRNAs, pseduogenes) and protein-coding genes from the miRNA-mediated (ceRNA) regulatory networks

3. RegRNA2.0：

an integrated web server for identifying functional RNA motifs and sites

示例結合分析：以H19為例

lncRNABase：

1. 打開(kāi)主頁(yè)面

2. 在“l(fā)ncRNA Gene Symbol”對話(huà)框中輸入H19

3. 點(diǎn)擊“search”，查詢(xún)即可獲取

• 1 . 生物信息學(xué)概述
• 2 . 生物信息學(xué)相關(guān)數據庫
• 3 . 轉錄因子相關(guān)數據庫
• 4 . miRNA與mRNA結合預測
• 5 . LncRNA與miRNA結合預測
• 6 . LncRNA與蛋白RNP結合預測